1.4 – ENZYMES
COURSE DESCRIPTION
Learning Outcomes: Candidates should be able to:
- Explain the mode of action of enzymes in terms of an active site, enzyme-substrate complex, lowering of activation energy and enzyme specificity using the lock-and-key and induced-fit hypotheses
- Investigate and explain the effects of temperature, pH, enzyme concentration and substrate concentration of an enzyme-catalysed reaction by measuring rates of formation of products (eg. Measuring gas produced using catalase) or rate of disappearance of substrate (eg. Using amylase, starch and iodine)
- Describe the structure of competitive and non-competitive inhibitors with reference to the binding sites of the inhibitor
- Explain the effects of competitive and non-competitive inhibitors (including allosteric inhibitors) on the rate of enzyme activity
Outline
- Introduction
- Enzymes are catalysts
- Properties of enzymes
- Structure of enzymes
- Nomenclature and classification
- Mode of Action of Enzymes
- Formation of enzyme-substrate complex
- How enzyme lowers activation energy
- Enzyme specificity
- Time Course of an Enzyme-catalysed Reaction
- Measuring rate of product formation
- Measuring initial rate of reaction
- Measuring rate of substrate usage
- Factors Affecting Rate of Enzymatic Reaction
- Substrate concentration
- Enzyme concentration
- Temperature
- pH
- Enzyme Kinetics
- Vmax and Km
- Enzyme Inhibition
- Types of inhibition
- Competitive and non-competitive inhibition
- Allosteric regulation
- Reversible and irreversible inhibition
- Control and Regulation of Metabolic Pathways (Flipped Classroom)
- Location of enzymes
- Advantages of metabolic pathways catalysed by enzymes
- End product inhibition
Requirements
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What is the target audience?
- This course is intended for anyone interested in learning to master his or her own body.
- This course is aimed at beginners, so no previous experience with hand balancing skillts is necessary
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CURRICULUM
Section 1: Introduction to Handstands
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Welcome to your second lesson review.
Here we will talk about your purposes on course and goal you want to reach
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Section 2: Reference Material, Moodboards and Mind Mapping
Wrist Strengthening
While your wrists will certain get stronger from practice and grow accustomed to the stress of the skill, a basic amount of wrist strengthening exercises for several weeks can only help things. I’d recommend working wrist curls and reverse wrist curls for around 6-10 reps for 3 sets. I also strongly recommend trying some sledgehammer levering. Work in 2-3 sets of 3-5 reps. In particular, exercises 1 and 3 are fantastic for building wrist strength and they are much harder than they look! Start with them to get the hang of sledgehammer work before you progress to the other two exercises. I don’t want somebody putting a hole through their floor or their face because they rushed things!
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This test will reveal how well you are acquiring the new material based on a short test of twenty questions and a brief written assignment.
Section 3: Sketching out Ideas
- The main objective to the sketching process is to generate super rough thumbnail sketches of what we feel best visually communicates the highlighted words from our mind maps.
- Take as much time as you need for this step — this might be 10 minutes or it might be 10 days.
- Personally, I like to work quickly and try not to analyze or elaborate too much.
- Now, that doesn't mean you should only create a handful of sketches.
- Even though this step only took a couple of hours, I was still able to put over 100 thumbnails on paper.
- The whole point of this process is to flush out the bad ideas and narrow down the good ones until we find that one layout that really speaks to us.
- Also, keep in mind the project brief and have your list in front of you as a reference to avoid getting sidetracked.
- Remember—detail is not needed. Simply flush out the bad ideas and find a great direction.
- Once I feel I have a good direction with the sketches, I'm now ready to take a quick photo with my phone and import it into Illustrator.
This test will reveal how well you are acquiring the new material based on a short quiz of ten questions and a short writing assignment.
Section 4: Conclusions and Evaluation
This final lecture will evaluate expectations and real gained knowledge and experience. Lecturer will share some self-development sources for further independent training of course participants.
Every course participant will be given a personalised practical task for creating branding logo design for real companies.
Every course participant will have to present his final assessment task result in his chosen individual manner to the audience.
About Instructors
PAUL HUNTINGTON BERNARDO
Healthcare & Life Sciences Strategy Consultant
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